Forensic Dental Identification Methods

BY SIFS India | April 19, 2023

Forensic Dental Identification Methods

Forensic Odontology is ever evolving advancing branch of dentistry. It is a subspecialty of dentistry concerned with diagnostic and therapeutic examination and evaluation of injuries to jaws, teeth, and oral soft tissues. It also helps in the identification of individuals, especially casualties in criminal investigations, mass disasters, sexual assaults, child abuse cases, and personal defence situations. The term “forensic” implies “court of law.” It has been defined as that branch of dentistry in the interest of justice. The principal basis of dental identification is that no two oral cavities are alike and the teeth are different in every individual. The dental evidence of the deceased recovered from the scene of the crime/occurrence is compared with the ante-mortem records for identification. Dental features such as tooth shape and size, restorations, pathologies, missing teeth, position of the tooth and other dental anomalies give every individual a unique identity. 


According to Federation Dentaire Internationale (FDI) Forensic Odontology is defined as “that branch of dentistry which is concerned in the interest of justice, deals with handling and examination of dental evidence, with the proper evaluation and presentation of dental findings.”

Based on the Field of Activity Avon classified Forensic Odontology into three types which include Civil, Criminal and Research Forensic Odontology.

Table 1 - Types of Forensic Odontology




It is concerned with mass disasters, malpractice and different frauds.

It is concerned with the identification of a person from their dental remains.

It is concerned with forensic odontology training for dental and medical professionals.

Such as in cases of airline accidents, train accidents, earthquakes, teen marriages and accident victims.

Such as in cases of suicide, rape, and homicides through different methods like rugoscopy, cheiloscopy.

Such as in crime and police departments.


6Personal Identification of dead person is important for legal, financial, social and, humanitarian reasons. Various methods of identification can be traditional methods like recognizing individual body, and property such as jewelry and clothing. Physical features of deceased may also help in identification of individual such as previous scar or fractures, but physical features may change over time.7 Dental identification like other hard tissues it is mostly preserved even after death. The dental status of an individual changes throughout life and combination of decayed, missing and filled teeth can be measured at any point of time.


Figure 1 -  Showing Anterior Teeth Fractured and Discolored after Car Crash and Fire

Dental Identification

Figure 2 - Chart showing the process of Comparative and Reconstructive dental identification

Comparative Dental Identification

It is used to compare and confirms that the remains of a descendant and that of the person represented by ante-mortem dental records are of the same individual. 

The steps of identification are as follows:

• Oral autopsy

• Obtaining dental records

• Comparing post and ante-mortem dental data

• Writing a report and drawing a conclusion.

A) Oral Autopsy

It is also called necropsy or postmortem, it involves an examination of a deceased person with the help of the dissection of different organs as to determine the cause of death of that person. The detailed oral examination is an important part of the postmortem procedure and all information pertaining to the body is entered into the modified Interpol postmortem dental odontograph.

Figure 3 - Pink Form for Collection of Dental Status from Human Remains

B) Obtaining Dental Records

As name suggests, it contains complete information on dental status of an individual during their lifetime. The dental record contains information in the form of radiographs, dental charts, casts and photographs. So, all available dental record of the person must be entered onto the modified Interpol antemortem odontography.


Figure 3 - Yellow Form for Collection of Dental Status of Missing Person

C) Comparing Postmortem and antemortem Dental Data

This includes a comparison between post-mortem and dental records that are available, features that are compared include bony structures, the morphology of tooth and dental restorations.

D) Writing Report and Drawing Conclusion

The AFBO has defined the categories for a dental identification

• Positive Identification: the ante mortem and post mortem features is correspondent to each other giving sufficient detail to establish that they are from same individual.

• Possible Identification: when the ante mortem and post mortem features are concordant but there is lack of quality in either ante mortem or postmortem features, so it is not possible to confirm dental identification.

• Insufficient Evidence: when the available ante mortem and post mortem information is insufficient to form a conclusion.

• Exclusion: when the ante mortem and post mortem data is clearly irreconcilable.

Reconstructive Identification

A) Identifying Ethenic Origin from Teeth

Each individual is unique in feature so defining a person’s race from dentition is a difficult task,but some dental features are more prominent in one group than the other group and these features only helps in identifying the individuals racial or ethnic origin. Broadly there are three major races, which are Caucasoid, Mongoloid, and Negroid.

Caucasoid mostly have narrow v-shaped arch and crowding of teeth, the anterior teeth of Caucasoid look chisel-shaped with small lingual surface, another common feature is cusp of carabellei present on mesiopalatal cusp of maxillary permanent first molars.

Negroids are characterized by having small size teeth with some spacing between them, midline diastema is also commonly seen. Third molars are usually present and other feature usually seen is increase incidence of supernumerary teeth.

In Mongloids lingual surface of incisors have fusion with lateral or marginal ridges which forms raised cingulum and deep lingual fossa. Also ridges fades towards incisal portion of teeth forming shovel and scoop shape appearance.

B) Sex Determination

Figure 4 - Methods of Sex Determination by Dental Evidence

1. Clinical Method

a) Hard Tissue Analysis

• Tooth Size: 10Mesidistal and buccolingual dimensions of tooth can be used for sex determination. There is difference in male and female tooth crown dimension in both permanent and deciduous dentition. Mandibular canine shows greatest difference in dimension with larger teeth in males than females.

• Canine Dimorphism: Permanent canine teeth and their inter canine distance helps in sex determination through dimorphism. The dimensions of canine teeth have been studied by methods, such as the measurement of linear dimensions such as mesiodistal width, Buccolingual width and inciso-cervical height, Fourier analysis , Moire topography.

• Root Length and Crown Diameter: 11Sex determination can be done with eighty percent accuracy by measuring root length and crown diameter by using an optical scanner and radio grammatic measurements. 

Another index, is the "mandibular canine index". Using the mesiodistal dimension of the mandibular canines, these researchers obtained the formula:

[(Mean m-d canine dimension + (Mean m-d canine dimension in female + standard deviation [SD]) in males − SD)]/2.

• Dental Index: Aitchison presented “incisor index” (Ii), which is calculated by the formula Ii = (MDI2/MDI1) ×100, where MDI2 stands for the maximum mesiodistal diameter of the maxillary lateral incisor and MDI1 stands for the maximum mesiodistal diameter of the central incisor. This index is higher in males, confirming the suggestion of Schrantz and Bartha that the lateral incisor is smaller than the central incisor in females.

• Tooth Morphology: It includes some features of tooth related to its structure such as distal accessory ridge, number of cusp in mandibular first molar can be used in sex determination

b) Soft Tissue Analysis

The analysis of soft tissue includes the study of lip prints (cheiloscopy) and study of palatal rugae patterns (rugoscopy).

Cheiloscopy: The word chelios is Greek word which means lip. Cheiloscopy is forensic method used in the study of lip prints. Lip prints can be identified at sixth week of intrauterine life and lip prints do not change after that. 

 Suzuki and Tsuchihashi’s Classification:

• Type I: Clear-cut grooves that runs vertically across the lip

• Type I′: The grooves are straight but disappear half-way and do not cover the complete breadth of the lip

• Type II: The grooves are branched

• Type III: The grooves intersect

• Type IV: The grooves are reticulate

• Type V: Undetermined.


Figure 5 -  Shows Lip Print Classification

Rugoscopy: Palatal rugoscopy or rugoscopy is the study of the pattern on the palatal rugae to identify a person. Palatal rugoscopy was first given by Trobo Hermosa, in 1932. It is helpful in forensics due to its internal position, stability, and perennity.

The rugae pattern is classified based on their length, shape, direction, and unification, proposed by Lysell and later modified by Thomas and Kotze

2. Microscopic Method

13Use of Barr method for sex determination can be done in conditions like fire and bombing where other methods cannot be used, as Barr bodies are stable at higher temperatures. When chromosomes are stained with quinacrine mustard, they fluoresce differentially along their length when seen under ultraviolet light. Barr bodies, are small well defined bodies found in nuclei of cells in females and are stained intensely by nuclear dyes. Duffy and coworkers in 1991 examined human dehydrated pulps from extracted teeth to assess sex chromatin from fibroblasts in artificially mummified and heated pulp tissues and they discovered that there is high sex chromatin stability.

3. Advanced Method

• DNA Analysis of Teeth: Molecular analysis of DNA is one of the best way for sex determination.DNA can be extracted by various methods such as by cryogenic grinding or by opening of root canals and scraping the pulp area and then it is analyzed by methods like Polymerase chain reaction (PCR), microarrays.14PCR works by amplifying small quantities of relatively short targeted sequences of DNA using oligonucleotide primers and thermostable Taq DNA Polymerase.

Sex Determination from Enamel Protein; Amelogenin or AMEL is an important protein in human enamel. It has different patterns of nucleotide sequence in both males and females. AMEL protein in females is located on the X chromosome and for male it is located on Y chromosome. Females have two identical AMEL genes and males have two non identical AMEL genes. This helps in determination of sex with small DNA sample.

Age Estimation from Dentition

Dental age estimation in Children and Adolescents

Depending on the presence or absence Mamelons present on the incisal edge of the permanent incisor teeth. The presence or absence of mamelons helps to differentiate between primary and permanent dentition. They found that more mamelons present in the first decade of life and decrease with increasing age. They are more prominent in permanent maxillary central incisor and more persistent in females than males.

Depending on the presence of teeth Schour and Massler modified Kronfeld's table of development and chronology of the growth of human teeth. Schour and Massler studied the development of deciduous and permanent teeth, from 4 months to 21 years of age and published the numerical development charts for them. It has three characteristics: Teeth that have erupted, amount of resorption of roots of primary teeth, amount of development of permanent teeth.

Demirjian’s Method

It is most common method used for dental age estimation of a person lying in the age group of 2–20 years. It consists of ten developmental staging from 0 to 9, and each staging has its own maturity score for boys and girls separately. The average sum should be hundred for all the teeth. The standard error rate in the Indian population of Demirjian’s method is ±1.17 for male and ±1.6 for female. The mandibular arch was selected due to the better quality of image as it is not superimposed by dental and cranial anatomy.

Gustafson's method

The first technique for age estimation was given by Gosta Gustafson in 1947 and 1950. This method is a morph histological method and it is applicable on single-rooted teeth only. The age changes are:

• Attrition of the enamel (A)

• Secondary dentin deposit (S)

• Alteration/recession of periodontal ligament (P)

• Cementum apposition (C)

• Root resorption (R)

• Transparency/translucency of dentin (T)

An + Pn + Sn + Cn + Rn + Tn = total score (Y) (n = score of individual criteria)

An increase in total score (Y) corresponds linearly with increase in age.


Forensic odontology is very essential field of dentistry as it helps in age and gender determination during mass destructions and it can also help in process of identification of a person by a forensic investigator in the case of child abuse, nuclear bomb explosions and crime investigations. Thus, forensic odontologist may play an important role in identification of an individual. Each practitioner has a responsibility to maintain legible and legally acceptable records and help forensic odontologist in investigation process whenever necessary.

Learning at SIFS India

To learn more, visit the Sherlock Institute of Forensic Science India where the team of expert professionals and certified faculty provides you with a great opportunity to learn forensics by attending different forensic events organized from time to time. Also, you can learn by registering yourself in forensic courses and training programs offered by SIFS India both in online and offline mode. You can also visit our YouTube channel for all the events recoding. 

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Note - The figures and images used in this blog are only for educational purposes.