Challenges in DNA Profiling Quiz Crafted By-
Vanshika
Introduction to Challenges in DNA Profiling
Rapid advancements in forensic DNA technology has resulted in its increasing use to resolve crime cases, particularly in the detection of low-level DNA traces. This has been made possible by the increasing sensitivity of STR typing kits. Low-template DNA analysis requires careful consideration of the derived stochastic variations that lead to heterozygote imbalance, allele drop-out and increased detection of background contamination. The relevance of the evidence and the probative value of the DNA profile are important issues in the evaluation of forensic evidence.
Let's look at the questions and their answers.
Ques 1. In SDS-PAGE electrophoresis, the role of SDS is to
a) Bind proteins
b) Denature proteins
c) Identify proteins
d) Isolate proteins
Answer-b) Denature proteins
Ques 2. The DNA Profiling was invented in
a) 1955
b) 1965
c) 1975
d) 1985
Answer- d) 1985
Ques 3. Fluorescent labelling of nucleic acids is usually carried out by-
a) Reduction reactions
b) Oxidation reactions
c) Enzymatic reactions
d) Duff reactions
Answer- c) Enzymatic reactions
Ques 4. The NCBI Gen Bank use which of the following Information retrieval tool?
a) Text search
b) Seqln
c) Entrez
d) STAG
Answer- d) STAG
Ques 5. Which of the following factor(s) affect the process of DNA extraction?
a) Time, Cost and Toxicity of the chemicals employed
b) Time, Toxicity of the chemicals employed
c) Time and Cost of the process
d) None of the Above
Answer- a) Time, Cost and Toxicity of the chemicals employed
Ques 6. DNA extraction from charred samples is challenging due to -
a) Inhibitor derived from the Maillard reaction
b) Production of various substances
c) Interaction between protein and carbohydrates
d) Enzymatic inhibitors
Answer- a) Inhibitor derived from the Maillard reaction
Ques 7. Which of the following is a thermo-stable DNA polymerase?
a) Taq polymerase
b) Vent polymerase
c) Pfu polymerase
d) All of these
Answer- d) All of these
Ques 8. A challenge of DNA extraction from blood is
a) Variability in Sample Size
b) Variability in Sample Volume
c) Variability in DNA quantity
d) None of the above
Answer- d) None of the above
Ques 9. Which of the following is excellent for preserving tissue structures, but can introduce all sorts of DNA damage?
a) Formalin
b) EDTA
c) Both a and b
d) Neither a nor b
Answer- a) Formalin
Ques 10. DNA staining is done by
a) Crystal violet
b) Giemsa staining
c) Methylene blue
d) Feulgen staining
Answer- d) Feulgen staining